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Table 2 Validation of selected genes by quantitative real time PCR

From: Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation

Pathways Genes Microarray QPCR
   NA16 Colony Tumor NA16 Colony Tumor
Hypoxia Hif1a NC 4.71 2.08 0.51 1.60 1.78
  Stc1 55.90 NC 55.60 873.10 1.01 347.26
  Vegfa -2.5 3.73 NC 10.56 1.37 6.59
  Vhl 3.68 NC NC 6.45 1.12 1.80
  Ddit3 8.45 2.14 4.64 11.31 1.52 1.00
  Egln3 7.72 12.18 11.58 68.12 22.16 25.46
Glycolysis/Gluconeogenesis Pfkm 3.60 -3.8 NC 4.29 1.32 2.35
  HK2 4.78 NC NC 29.02 1.36 3.05
  Pdk1 9.11 NC -2.75 13.00 1.00 2.00
  Adh1 10.76 66.9 47.76 4.44 216.77 135.30
  Aldh3a1 10.52 3.49 -2.36 3.36 18.38 1.04
  Slc2a1 5.80 NC 2.11 27.86 1.64 1.78
Tumor formation/
metastasis
Spp1 NC 22.24 145.20 6.19 64.00 139.10
  Mmp3 NC 33.52 87.30 0.03 0.04 6.68
  Egfr 2.43 -2.46 NC 0.01 0.01 0.10
  Rb1 2.48 3.17 3.60 0.69 1.12 2.64
  1. Total RNA was collected from F111-NA16, Colony and tumour cells and validated for the selected genes of Hypoxia, glycolysis/gluconeogenesis and tumour formation/metastatsis from microarray data and were compared with control[F111-A16]. Genes showed the same trend in regulation in the qPCR and microarray experiments, but not necessarily by the same fold change. No change (NC) refers to a gene whose expression was below twofold with p Value less than 0.05 for the microarray.