Impact of glass slide temperature on cell transfer efficiency and RNA extraction yield. Frozen tissues cut at 8 μm and transferred on glass slides that have been placed at room temperature (A) or chilled and kept at 4°C, before transfer (D). As evidenced by the number of cells captured (C and F) and the RNA yield, given under each cap (C and F) the efficiency of capture was very low for the slides placed at room temperature. A/D: Cresyl violet® stained slides before LCM; red arrows indicate the thermoplastic film stuck on epithelial cells to be captured. B/E: mammary tissue section remaining on the glass slide after LCM. C/F: microdissected mammary epithelial cells transferred on the LCM cap. Magnification: ×60.