Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection

Table 2 Primers used in this study.

RPS24 F	TTT GCC AGC ACC AAC GTT G
RPS24 R	AAG GAA CGC AAG AAC AGA ATG AA
PPIA F	TGA CTT CAC ACG CCA TAA TGG T
PPIA R	CAT CAT CAA ATT TCT CGC CAT AGA
CSN1S2 F	CTG GTT ATG GTT GGA CTG GAAAA
CSN1S2 R	AAC ATG CTG GTT GTA TGA AGT AAA GTG
Krt14 F	CCC AGC TCA GCA TGA AAG C
Krt14 R	AGC GGC CTT TGG TCT CTT C
CD3e F	ACG CTGT ACC TGA AAG CAA GA
CD3e R	AAT ACA CCA GCA GCA GCA AG
CD68 F	GAT CTG CTC TCC CTG AAG CTA CA
CD68 R	CAT TGG GAC AAG AGA AAC TTG GT
FASN F	ACA GCC TCT TCC TGT TTG ACG
FASN R	CTC TGC ACG ATC AGC TCG AC

Each pair of primers amplifies the target cDNA in its 3' region. Primer pairs were designed with the Primer Express Software v2.0 (Applied Biosystems) except for RPS24 primers which were manually designed.

ISSN: 2661-8850