Figure 3

Reducing the level of hRev7 in human fibroblasts results in a BPDE-induced delay in progression through the cell cycle. Cell strains with reduced levels of hRev7 (2-2 and 2-6), their parental cell strain (P), and a vector control transfectant (VC) were synchronized at the G1/S border and treated with BPDE for one h immediately after release from synchrony. Cells were harvested, fixed, and stained with propidium iodide for analysis of DNA content by flow cytometry. The distribution of cells in each phase in the cell cycle 0 h, 4 h, 8 h, 12 h, 16 h, 20 h, and 24 h post-BPDE treatment are depicted.