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Figure 4 | BMC Cell Biology

Figure 4

From: Long term culture of mesenchymal stem cells in hypoxia promotes a genetic program maintaining their undifferentiated and multipotent status

Figure 4

Culture in hypoxia enhanced the potential of MSC to differentiate in adipocytes (A) and osteocytes (O). MSC were cultured in hypoxia or normoxia until P2, and shifted to adipocyte- or osteocyte-specific differentiation conditions respectively for 2-4 more weeks. The aspect of differentiated colonies under hypoxia is shown (A). In 2 independent experiments (B), MSC were harvested at P2, RNA from normoxic (N) or hypoxic (H) cells was extracted, reverse-transcribed and amplified by PCR with primers specific for control GAPDH and the indicated genes, representative of adipocytic (LPL, PPARĪ³) and osteocytic (ALPL, Runx2) lineages. The same cells were grown in osteocytic and adipocytic-specific medias and colonies were counted and compared to the numbers of CFU-F generated in MSC-specific medium (C).

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