Long term culture of mesenchymal stem cells in hypoxia promotes a genetic program maintaining their undifferentiated and multipotent status

Table 1 Ten first down and upregulated genes at P0 in hypoxia.

Gene	Fold expression H/N	Genbank Accession	Putative Function
Down Regulated
RRM2	5.94	NM_001034	Ribonucleotide Reductase
XRCC2	5.80	CR749256	X Ray damage DNA Repair
KIF24	5.16	AK001795	Kinesin: chromatid assembly
POLQ	4.99	AF090919	DNA polymerase theta
E2F8	4.95	NM_024680	Cell cycle progression
FANCD2	4.93	NM_001018115	DNA Repair
ESCO2	4.86	NM_001017420	Sister chromatid cohesion
AURKB	4.74	NM_004217	Chromosome segregation
CENPN	4.48	AK023669	Binding to Centromeres
MKI67	4.46	NM_002417	Cell proliferation
Up Regulated
TCF1	6.96	NM_000545	Hepatic Transcription Factor
LEP	6.39	NM_000230	Metabolism, apoptosis, angiogenesis
ANGPT2	5.69	NM_001147	Antagonise vascular remodelling
ZP1	5.47	NM_207341	Sperm binding to zona pellucida
VWF	5.41	NM_000552	Platelet binding to endothelium
GIMAP4	5.37	NM_018326	T-cell development, Tumor suppressor ?
CD93	4.56	NM_012072	Intercellular adhesion, clearance apoptotic cells
PLVAP	4.25	NM_031310	Adhesion of Vascular Endothelial cells ?
ESAM	4.24	NM_138961	Adhesion of Endothelial cells
PCDH17	4.14	NM_001040429	Cell-cell connexions in the brain

Comparative analysis of transcriptome from MSC at P0 in hypoxia versus normoxia. Mean from 3 distinct samples.

ISSN: 2661-8850