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Table 5 List of primers used for qPCR (GAPDH is used as a calibrator)

From: Long term culture of mesenchymal stem cells in hypoxia promotes a genetic program maintaining their undifferentiated and multipotent status

Gene Product Primers Product Size (bp)
HoxA11 (NM_005523.5) Fw: 5'-TTGAGCATGCGGGACAGTT-3'
Rv: 5'-GTACCAGATCCGAGAGCTGGAA-3'
87
OxCT2 (NM_022120.1) Fw: 5'-GAGTTCAACGGCGACCACTT-3'
Rv:5'-GCGCTTCTCCTGAAGACCA-3'
110
V-KIT (NM_000222.2) Fw: 5'-GGCGACGAGATTAGGCTGTT-3'
Rv: 5'-CATTCGTTTCATCCAGGATCTCA-3'
77
CCL2 (NM_002982.3) Fw: 5'-ACTCTCGCCTCCAGCATGAA-3'
Rv: 5'-GGGAATGAAGGTGGCTGCTA-3'
72
CX3CL1 (NM_002996.3) Fw: 5'-TGACATCAAAGATACCTGTAGC-3'
Rv: 5'-CTCGTCTCCAAGATGATTGC-3'
88
WNT4 (NM_030761.4) Fw: 5'-AGCAACTGGCTGTACCTG-3'
Rv: 5'-CTGGATCAGGCCCTTGAG-3'
87
GAPDH (NM_002046.3) Fw: 5'-CGCTCTCTGCTCCTCCTGTT-3'
Rv: 5'-CCATGGTGTCTGAGCGATGT-3'
81