Table 5 List of primers used for qPCR (GAPDH is used as a calibrator)
Gene Product | Primers | Product Size (bp) |
|---|---|---|
HoxA11 (NM_005523.5) | Fw: 5'-TTGAGCATGCGGGACAGTT-3' Rv: 5'-GTACCAGATCCGAGAGCTGGAA-3' | 87 |
OxCT2 (NM_022120.1) | Fw: 5'-GAGTTCAACGGCGACCACTT-3' Rv:5'-GCGCTTCTCCTGAAGACCA-3' | 110 |
V-KIT (NM_000222.2) | Fw: 5'-GGCGACGAGATTAGGCTGTT-3' Rv: 5'-CATTCGTTTCATCCAGGATCTCA-3' | 77 |
CCL2 (NM_002982.3) | Fw: 5'-ACTCTCGCCTCCAGCATGAA-3' Rv: 5'-GGGAATGAAGGTGGCTGCTA-3' | 72 |
CX3CL1 (NM_002996.3) | Fw: 5'-TGACATCAAAGATACCTGTAGC-3' Rv: 5'-CTCGTCTCCAAGATGATTGC-3' | 88 |
WNT4 (NM_030761.4) | Fw: 5'-AGCAACTGGCTGTACCTG-3' Rv: 5'-CTGGATCAGGCCCTTGAG-3' | 87 |
GAPDH (NM_002046.3) | Fw: 5'-CGCTCTCTGCTCCTCCTGTT-3' Rv: 5'-CCATGGTGTCTGAGCGATGT-3' | 81 |