Aurora-A gene copy numbers, mRNA and protein expression in normal esophageal epithelial cells and esophageal cancer cells. A. FISH analysis of AURKA (red signals) and Chromosome 20 (CEP20; green signals). Bar = 20 μm, all panels are in the same magnification. B. Relative Aurora-A mRNA expression was determined by qRT-PCR (mean ± standard deviation of three independent experiments). C. Photograph of Aurora-A/phospho-T288 and Aurora-A protein expression by immunoblot analysis, representative for 3 independent experiments. β-Actin served as loading control.