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Figure 2 | BMC Cell Biology

Figure 2

From: Occurrence of multipolar mitoses and association with Aurora-A/-B kinases and p53 mutations in aneuploid esophageal carcinoma cells

Figure 2

Aurora-A gene copy numbers, mRNA and protein expression in normal esophageal epithelial cells and esophageal cancer cells. A. FISH analysis of AURKA (red signals) and Chromosome 20 (CEP20; green signals). Bar = 20 μm, all panels are in the same magnification. B. Relative Aurora-A mRNA expression was determined by qRT-PCR (mean ± standard deviation of three independent experiments). C. Photograph of Aurora-A/phospho-T288 and Aurora-A protein expression by immunoblot analysis, representative for 3 independent experiments. β-Actin served as loading control.

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