Aurora-B gene copy numbers, mRNA and protein expression in normal esophageal epithelial cells and esophageal cancer cells. A. FISH analysis of AURKB (red signals) and Chromosome 17 (CEP17; green signals). Bar = 20 μm, all panels are in the same magnification. B. Relative Aurora-B mRNA expression was determined by qRT-PCR (mean ± standard deviation of three independent experiments). C. Photograph of Aurora-B/phospho-T232 and Aurora-B protein expression by immunoblot analysis, representative for 3 independent experiments. β-Actin served as laoding control.