Occurrence of multipolar mitoses in normal esophageal epithelial cells and esophageal cancer cells. The figure provides representative pictures of HE stainings as well as Aurora-A and pericentrin indirect immunofluorescence analyses of EPC-hTERT, OE21, Kyse-410, OE33 and OE19 cell lines. Aurora-A staining (green) and pericentrin staining (green), each with counterstaining of DNA by DAPI (blue). Bar = 20 μm, all panels are in the same magnification. Note that the mitotic index and occurrence of multipolar mitoses were quantified according to Aurora-A indirect immunofluorescence of three independent experiments, with multipolar mitoses (arrows) most frequently observed in OE33 and OE21 cells (see Table 2 for quantification).