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Table 3 Summary of investigated parameters in normal esophageal epithelial cells and esophageal cancer cell lines.

From: Occurrence of multipolar mitoses and association with Aurora-A/-B kinases and p53 mutations in aneuploid esophageal carcinoma cells

   ESCC BAC
  EPC-hTERT OE21 Kyse-410 OE33 OE19
Aurora-A
Aurora A gene copies 2.0 ± 0.2 4.2 ± 0.5 9.1 ± 1.2 7.5 ± 0.9 3.9 ± 0.3
Centromere 20 signals 1.9 ± 0.3 4.1 ± 0.7 4.6 ± 0.9 7.2 ± 0.9 2.9 ± 0.4
Aurora-A mRNA 0.4 ± 0.1 0.8 ± 0.1 2.7 ± 0.3 1.3 ± 0.3 1.6 ± 0.1
Aurora-A protein ++ + +++ +++ +
Aurora-A/phospho T288 + + + (+) (+)
Aurora-B
Aurora-B gene copies 1.9 ± 0.4 3.0 ± 0.1 3.6 ± 0.8 2.9 ± 0.7 2.0 ± 0.2
Centromere 17 signals 2.0 ± 0.2 3.0 ± 0.1 3.8 ± 0.7 4.9 ± 0.7 3.9 ± 0.4
Aurora A mRNA 0.7 ± 0.0 1.2 ± 0.3 1.5 ± 0.5 0.7 ± 0.4 0.8 ± 0.2
Aurora-B protein (+) +++ ++++ ++ +
Aurora-B/phospho T232 (+) + + + -
p53
Mutations no mutation c.269C>T, c.270delC, p.S90fs31X c.1009C>T, p.R337C c.404G>A, p.C135Y c.928_930insA, p.N310fs26X
p53 protein (kDa) 53 ~14 (truncated) 53 53 ~40 (truncated)
p53 functionality + - +/- + +/-
Mitoses
Mitotic Index (%) 1.0 ± 0.0% 4.6 ± 1.2% 2.2 ± 0.4% 3.4 ± 2.2% 3.2 ± 1.9%
Multipolar mitoses (%) 0.3 ± 0.6% 7.7 ± 5.0% 6.3 ± 2.0% 13.8 ± 4.2% 1.0 ± 1.0%
  1. Quantitative data of each three independent experiments are summarized for FISH and q-RT-PCR (relative mRNA expression) analyses as well as for mitoses and multipolar mitoses (mean and ± standard deviation). Immunoblot analyses are depicted with minus (-) to increasingly positive (+ to ++++) protein expression. Identified p53 mutations and associated effects are listed.