Figure 4

Correlation of STAT3 nuclear translocation inhibition and cell death induction. A: Cell death induction in STAT3-decoy ODN-transfected (2 μg/ml) SW 480 and MCF-7 cells, controls: no addition, mutated STAT3-decoy ODN (mtODN, 2 μg/ml); resistance of the acute myeloid leukemia cells KG1 to STAT3-decoy ODN (2 μg/ml), controls: non-transfected and mutated STAT3-decoy ODN (mtODN)-transfected (2 μg/ml) cells. Dead cells, incorporating trypan blue, were counted using the V-cell apparatus. Values are expressed in % of dead cells. B: Expression of cyclin-D1 in non-transfected, STAT3-decoy ODN-transfected (2 μg/ml), Stattic-treated (20 μM), and mutated STAT3-decoy ODN-transfected (2 μg/ml) SW 480 cells. Expression was measured using qPCR. Data, normalized using cyclophilin expression (ΔΔct), are expressed as % of control.