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Figure 5 | BMC Cell Biology

Figure 5

From: A STAT3-decoy oligonucleotide induces cell death in a human colorectal carcinoma cell line by blocking nuclear transfer of STAT3 and STAT3-bound NF-κB

Figure 5

Involvement of STAT1 expression level in STAT3-decoy ODN-induced cell death. Colon carcinoma SW 480 cells transduced either with control virus (black histograms) or with STAT1-specific shRNA (white histograms). A: Detection of STAT1 by western blotting of control cells (1), cells transduced with an empty virus (Ev) (lane 2), and with a STAT1-specific shRNA lentivirus (3). Below are shown the detection of STAT3, the NF-κB (p50 subunit) and actin in the same extracts. B: Detection of dead cells by trypan blue exclusion counting. Control non-treated (black histograms) and STAT1-shRNA-treated cells (white histograms) were either not treated or transfected with STAT3-decoy ODN (ST3dODN) (48 h), mutated STAT3-decoy ODN (mtdODN) (48 h) or IFNγ (100 ng/ml, 48 h). Data are expressed in % of dead cells.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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