Figure 7
The CRM1 inhibitor leptomycin B and the phosphatase inhibitor vanadate reverse inhibition of STAT3 nuclear translocation by STAT3-decoy ODN. A: Amounts of nuclear STAT3 following treatment of cells with leptomycin (LMB) or vanadate. Cells were either not treated (1) or treated with LMB (10 ng/ml, 3 h) (2), sodium vanadate (100 μM, 2 h) (3), or both (4); for clarity, only nuclear extracts (N) are shown. B: Effect on STAT3 nuclear localization of cells' simultaneous treatment with STAT3-decoy ODN, LMB, and vanadate. Cells were either not transfected (1) or transfected with STAT3-decoy ODN alone (2 μg/ml) (4), STAT3-decoy ODN (2 μg/ml) and vanadate (100 μM) (2), or STAT3-decoy ODN (2 μg/ml) and LMB (10 ng/ml, 3 h) (3). Only nuclear extracts are shown (N). C: Quantitative analysis of nuclear STAT3 from several experiments identical to those shown in A and B. STAT3 amounts quantified by scanning are expressed relative to control (in %). Conditions were: STAT3-decoy ODN (ST3dODN) and vanadate (VO4), STAT3-decoy ODN and LMB, STAT3-decoy ODN alone, vanadate, LMB, vanadate and LMB. Data are from at least three separate experiments.