Figure 8

Interaction of STAT3 with the importin/karyopherin complex is impaired by STAT3-decoy ODN. A: Co-immunoprecipitation of STAT3 with karyopherin α. Cells were lysed and immunoprecipitation performed with anti-STAT3 (1:100) (3) or with anti-karyopherin α (1:40) (4) antibodies. A control experiment was performed with non-relevant immunoglobulin (IgG, 1:50) (2). After separation on gel and transfer, the membrane was probed with anti-karyopherin α (1:400) or anti-STAT3 antibody (1:1000). A control cell lysate was loaded on the gels (1). B: Intra-cellular pull-down assays with the biotinylated STAT3-decoy ODN. Cells were transfected with biotinylated STAT3-decoy ODN (6 h) and complexes collected with avidin agarose and analyzed by western blotting. Control SW 480 cell lysate (1), STAT3-decoy ODN (ST3dODN) pull-down (2). Membranes were probed with antibody to STAT3 and reprobed with antibody to karyopherin α.