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Figure 4 | BMC Cell Biology

Figure 4

From: Quantitative digital in situ senescence-associated β-galactosidase assay

Figure 4

pH effect on BGAVs. A. FSE cells at PD 34 stained at pH 4.0. All cells demonstrated some degree of staining. B. BGAVs are inversely related to the pH at which the reaction was carried out. Staining was performed on FSE cells at PD 42 under different pH conditions. C. The effect of increasing PDs on BGAVs obtained at pH 4.0. FSE cells at progressively increasing PDs were stained at pH 4.0 and analyzed for BGAVs. D. The effect of H2O2 on BGAVs obtained at pH 4.0. FSE cells were treated with progressively increasing H2O2 concentrations and BGAVs were determined at each concentration.

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