Figure 7
From: Quantitative digital in situ senescence-associated β-galactosidase assay
γ-H2AX foci in diabetic and non-diabetic mice. γ-H2AX staining was carried out for kidney sections at the 13-week time point for both control and diabetic (DM) mice. Red - γ-H2AX signals, Blue - DAPI nuclear staining. One hundred tubule cells from each sample were analyzed for the number of γ-H2AX foci present in the nucleus. The percentage of cells displaying 0-3 foci per nucleus appears in the graph (3 foci was the maximum number of foci detected per nucleus).