Overall morphology and differentiation does not appear to be affected in STC2Tgmice. (A, B) IHC for STC2 in WT (A) and STC2Tg(B) pancreatic islets shows that STC2 accumulates in both acinar (*) and islet (I) tissue only in the STC2Tgmice. Magnification bar = 27 μm. Tissue was counterstained with hematoxylin to reveal tissue morphology. (C, D) Hematoxylin and eosin staining comparing general histology of pancreatic tissue in WT (C) or STC2Tg(D) pancreata. Magnification bar = 60 μM; I - islet. IF analysis for MIST1 (E, F), PDX1 (G, H) or GLUT2 (I, J) in WT (E, G, I) or STC2Tg(F, H, J) pancreata. Magnification bar = 54 μm. (K) Representative Western blot analysis for pancreatic differentiation markers amylase, MIST1, PDX1 and GLUT2.