Figure 2

Overall morphology and differentiation does not appear to be affected in STC2^Tgmice. (A, B) IHC for STC2 in WT (A) and STC2^Tg(B) pancreatic islets shows that STC2 accumulates in both acinar (*) and islet (I) tissue only in the STC2^Tgmice. Magnification bar = 27 μm. Tissue was counterstained with hematoxylin to reveal tissue morphology. (C, D) Hematoxylin and eosin staining comparing general histology of pancreatic tissue in WT (C) or STC2^Tg(D) pancreata. Magnification bar = 60 μM; I - islet. IF analysis for MIST1 (E, F), PDX1 (G, H) or GLUT2 (I, J) in WT (E, G, I) or STC2^Tg(F, H, J) pancreata. Magnification bar = 54 μm. (K) Representative Western blot analysis for pancreatic differentiation markers amylase, MIST1, PDX1 and GLUT2.