Systemic over-expression of STC2 ( STC2Tg) alters the PERK signalling pathway in pancreatic tissue. (A) Representative Western blot analysis for mediators of PERK signalling: pPERK, peIF2α, total (t) eIF2α, ATF4 and sXBP1 in wild type (WT) and STC2Tg(Tg), revealed decreased levels of pPERK, peIF2α and sXBP1, and increased amounts of ATF4 in STC2Tgmice. (B) Densitometry revealed that the ratio of peIF2α to total eIF2α is decreased in STC2Tgextracts relative to WT tissue (n = 3 animals; *p < 0.05). (C) Similar western blot analysis revealed increased accumulation of GADD34 and decreased accumulation of calcineurin in STC2Tgpancreatic protein extracts. No difference in calregulin accumulation was observed. Molecular weights (kDa) are provided.