Figure 6

Cell death is decreased during CIP in STC2^Tgtissue. TUNEL analysis revealed apoptosis (arrowheads) during CIP (B, D) but not saline injections (A, C) in WT (A, B) and STC2^Tg(C, D) pancreata. Magnification bar = 45 μM. (E) Quantification of TUNEL positive cells in WT and STC2^Tgtissue (n = 4 animals; letters indicate significant differences). (F-I) HMGB1 IF to identify necrosis revealed nuclear localization in saline conditions for WT (F; n = 3 animals) and STC2^Tg(H; n = 3 animals) tissue. After induction of pancreatitis, patches of cells with cytoplasmic, punctate HMGB1staining, indicative of necrosis, were observed in WT tissue (G; n = 4 animals) but not in STC2^Tgtissue (I; n = 4 animals). Magnification bar = 34 μM