Figure 4

3D co-localization of MMGL and its respective preys identified in the Y2H library screen. Representative images of live cell fluorescence microscopy showing co-localization of MMGL and the putative interactors identified in the Y2H library screen in differentiated H9C2 cardiac myocytes. (i) Individual GFP-tagged putative library screen interactors are seen as green fluorescence, as indicated by labels to the left of the row. (ii) dsRed-tagged MMGL expression in the same cell(s) are shown are red fluorescence. (iii) Co-localization of interactors and MMGL within these cell(s), generated from 3D vertical Z-stack images, are shown as yellow fluorescence. (iv) Overlay of images A-C with Hoechst H-33342 labelling of the nuclei (blue) for orientation purposes. The presence of yellow staining in each of the images in (iii) indicates that each of the respective preys colocalize with MMGL in differentiated H9C2 cardiomyocytes. Scale bar: 0.02 mm