Table 1 Interactors of the C1-C2 region of cMyBPC in the trisphospho-mimic state as identified by yeast 2-hybrid library screening
Clone # | Genomic Hit, Accession no (E-value) | In-frame ORF Protein Hit, Accession no (E-value) | Subcellular localization |
|---|---|---|---|
P205, P272, P403 | PDE4DIP, transcript 4 NM_001002810 (0.0) | Homo sapiens phosphodiesterase 4D interacting protein (myomegalin) NP_055459.3 (1e-70) | Cytoplasm, Cytoskeleton |
P182, P286, P267 | ACTC1 NM_005159 (0.0) | Homo sapiens actin, alpha, cardiac muscle 1 NP_005150 (1e-89) | Thin filament sarcomere |
P259 | ACTCB NM_001101 (0.0) | Homo sapiens actin, beta AAH23548.1 (8e-34) | Cytoplasm, cytoskeleton |
P62, P213, P319 | MYBPC3 NM_000256 (0.0) | Homo sapiens cardiac myosin binding protein C, NP_000247.2 (2e-26) | Thick filament; Sarcomere |
P2,P81 | Gcom1 transcript 12 NM_001018100 (0.0) | Homo sapiens GRINL1A combined protein NP_001018100.1, (2e-65) | Unknown |
P80 | FLNC NM_001458.2 (0.0) | Homo sapiens filamin C, gamma NP_001449.3 (2e-91) | Myofibrillar z-disks; actin cytoskeleton |
P175, P465 | CNN1 NM_001299.4 (0.0) | Homo sapiens calponin 1, basic, smooth muscle NP_001290.2 (1e-104) | Cytoplasm, cytoskeleton |
P11 | PARD3 NM_019619.2 (3e-121) | Homo sapiens par-3 partitioning defective 3 homolog NP_062565.2 (1e-24) | Plasma membrane; tight junctions |
P223 | CCT7 NM_001009570.1 (0.0) | Homo sapiens chaperonin containing TCP1, subunit 7 (eta) NP_001009570.1 (1e-83) | Cytoplasm |
P238 | SVIL NM_021738.1 (0.0) | Homo sapiens supervillin NP_003165.1 (1e-15) | Actin cytoskeleton, plasma membrane, nucleus |
P398 | FLJ21347 NM_022827.2 (0.0) | FLJ21347 NP_073738.2 (4e-101) | Unknown |