Figure 4

Dose-dependent reduction of expression of Cx43 protein by AGE-BSA, as detected by Western blotting. Note that a dose-dependent reduction is seen in cells treated with AGE-BSA for both 24 (A) and 48 hours (B). For each dose of treatment, the relative level of the total amount of Cx43 protein is shown in the histogram at the top of the blot. The relative expression levels of Cx43 protein significantly reduced to 61.2 ± 7.3% and 43.9 ± 8.8% after cells exposure to 500 μg/ml of AGE-BSA for 24 and 48 hours, respectively. *, p < 0.05 compared to the untreated cells.