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Figure 3 | BMC Cell Biology

Figure 3

From: Surface expression and limited proteolysis of ADAM10 are increased by a dominant negative inhibitor of dynamin

Figure 3

Activation of muscarinic M3 receptors increases constitutive release of sAPPα, but does not affect ADAM10 surface expression. A. HEK-M3 cells were transfected with ADAM10, APP695, or empty vector as indicated. After 48 h cells were pre-incubated for 1 h in serum-free DMEM, then incubated in fresh DMEM in the presence of carbachol (100 μ M) or vehicle control (water) for 2 h. Medium was collected, and cells were surface-biotinylated and lysed. Medium extracts were immunoblotted with 6E10 antibodies to sAPPα. Biotinylated proteins were immunoblotted with antibodies to APP or ADAM10. B. Lysates from vehicle-treated cultures were immunoblotted with antibodies to the APP C-terminus. C. Results were quantitated, normalized to control values, and expressed as means ± SEM from 4 or 5 experiments. *Significantly different from vehicle-treated APP695/vector transfectants. **Significantly different from carbachol-treated APP695/vector transfectants.

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