Figure 5

Stimulation of M3 receptors does not accelerate formation of the ADAM10 CTF. A. HEK-M3 cells were transfected with empty vector, ADAM10, or dyn I K44A, as indicated. After 48 h cells were pre-incubated for 1 h in serum-free DMEM, then incubated in fresh DMEM in the presence of carbachol (100 μ M) or vehicle control for 2 h. Cell lysates were prepared and subjected to immunoblot analysis. B. Levels of the most prominent ADAM10 CTF (~16 kDa) were quantitated, normalized to levels in vehicle-treated ADAM10/dyn I K44A transfectants, and expressed as means ± SEM from 3-4 experiments. *Significantly different from corresponding ADAM10 transfectants.