Figure 4

Co staining of Golgi markers GM130 and GCC88 in Drosophila testes. Testes squashes were triple labelled for GM130 (Golgi marker), GCC88 (TGN marker) and Hoechst (nuclei). Note the coincident labelling of GM130 and GCC88, seen most clearly in the large peripheral puncta in the spermatocytes (A) and the cone-shaped juxtanuclear acroblast in the 'onion stage' spermatids (B). Note that the staining of GM130 is slightly off-set relative to GCC88 as we would expect since they are on opposed sides of the Golgi stack. Bar, 20 μm