Figure 4

A putative membrane association domain and pore-lining residue are required for torA-ΔE localization in the NE. (A) Schematic of torA structure showing the location of amino-terminal ER targeting sequence, putative membrane association domain, together with the Y147, E171 and E302/3 (mutated by ΔE) residues. (B) Images show anti-GFP labeling of BHK21 cells expressing (left panels) "control" (GFP)torA-WT, (GFP)torA-ΔE or (GFP)torA-E171Q proteins, or (central column) these torA isoforms lacking residues 22 - 40 ("Δ22-40"), or (right column) torA isoforms containing the Y147A mutation ("+ Y147A"). Scale bars show 10 μm.