Figure 5

SUN1 depletion also reduces NE-localized torA-WT. The (GFP)torA-WT normally present in the NE of NIH-3T3 cells is reduced by SUN1 siRNA treatment. Images show anti-GFP labeling of NIH-3T3 cells expressing (GFP)torA-WT (green) and transfected with control (left) or SUN1 siRNA duplexes (right). Anti-LaminA/C labeling (red) highlights the position of the NE and lower panels show the merge (yellow) of (GFP)torA-WT and NE-marker signals, with less yellow signal present in the cell lacking SUN1. Scale bars show 10 μm.