Figure 5
From: Dynamic reorganization of flotillins in chemokine-stimulated human T-lymphocytes
Role of flotillin-F-actin interaction in flotillin cap formation in human T-lymphoblasts. Cells were transfected singly (upper panels) or together (lower panels) with a construct encoding for flotillin-2 lacking the F-actin binding domain (R1MCT-EGFP) and flo1-HA, as indicated. 4 hr after transfection cells were stimulated with 40 ng/ml SDF-1, fixed with PFA and the HA-tag was visualized by immunofluorescence staining using a murine monoclonal anti-HA antibody. Numbers in the panels indicate the fraction of cells with flotillin caps (mean ± s.e.m.) obtained in 3-4 independent experiments.