Dynamic reorganization of flotillins in chemokine-stimulated human T-lymphocytes

Table 3 Lateral mobility of the flotillin-2 mutant lacking the actin binding domain (R1MCT) expressed in freshly isolated T-lymphocytes without or with wild type flotillin-1-mCherry

Transfection	M_F - SDF-1 (n)	M_F + SDF-1 (n)
R1MCT	0.72 ± 0.05 (15)	0.79 ± 0.06 (14)
R1MCT/flo1-mCherry	0.65 ± 0.06 (14)	0.79 ± 0.05 (15)

FRAP analysis involving local bleaching of EGFP was carried out as described in the legend of Figure 6 with cells preincubated in the absence or presence of SDF-1 as indicated. The mobile fraction (M_F) was calculated for each individual cell and then averaged from a total of n = 14 - 15 cells observed in 3 independent experiments, as indicated (mean ± s.e.m.).

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