Skip to main content

Table 2 PCR primer pairs used amplify CCSP, sPLA2, cPLA2 and β-actin cDNA fragments of rat lung

From: Polydatin up-regulates clara cell secretory protein to suppress phospholipase A2 of lung induced by LPS in vivo and in vitro

Target Oligonucleotide sequence Tm (°C) bp
β-actin F: 5'-AGAGGGAAATCGTGCGTGAC-3' 60 195
  R: 5'-CCATACCCAGGAAGGAAGGCT-3'   
CCSP F: 5'-CGGACATCTGCCCAGGATTTCT-3' 60 209
  R: 5'-ACACAGAGGACTTGTTAGGAT-3'   
sPLA2 F: 5'-CCCCAAGGATGCCACAGATT-3' 60 201
  R: 5'-TTCCGGGCAAAACATTCAGC-3'   
cPLA2 F: 5'-GAAGTTTGCTCATGCCCAGACCT-3' 60 234
  R: 5'-TTCATAGAGCGCCTTCATCACACC-3'   
  1. F, forward primer; R, reverse primer; Tm, melting temperature.