Table 2 PCR primer pairs used amplify CCSP, sPLA2, cPLA2 and β-actin cDNA fragments of rat lung
Target | Oligonucleotide sequence | Tm (°C) | bp |
|---|---|---|---|
β-actin | F: 5'-AGAGGGAAATCGTGCGTGAC-3' | 60 | 195 |
| Â | R: 5'-CCATACCCAGGAAGGAAGGCT-3' | Â | Â |
CCSP | F: 5'-CGGACATCTGCCCAGGATTTCT-3' | 60 | 209 |
| Â | R: 5'-ACACAGAGGACTTGTTAGGAT-3' | Â | Â |
sPLA2 | F: 5'-CCCCAAGGATGCCACAGATT-3' | 60 | 201 |
| Â | R: 5'-TTCCGGGCAAAACATTCAGC-3' | Â | Â |
cPLA2 | F: 5'-GAAGTTTGCTCATGCCCAGACCT-3' | 60 | 234 |
| Â | R: 5'-TTCATAGAGCGCCTTCATCACACC-3' | Â | Â |