Table 3 PCR primer pairs used amplify CCSP and β-actin cDNA fragments of BEAS-2B cells
Target | Oligonucleotide sequence | Tm (°C) | bp |
|---|---|---|---|
CCSP | F: 5'- GAAACTCGCTGTCACCCTCACC-3' | 60 | 211 |
| Â | R: 5'-TTAATGATGCTTTCTCTGG-3' | Â | Â |
β-actin | F: 5'-CCTGTACGCCAACACAGTGC-3'; | 60 | 234 |
| Â | R: 5'-ATACTCCTGCTTGCTGATCC-3' | Â | Â |