Skip to main content

Table 3 PCR primer pairs used amplify CCSP and β-actin cDNA fragments of BEAS-2B cells

From: Polydatin up-regulates clara cell secretory protein to suppress phospholipase A2 of lung induced by LPS in vivo and in vitro

Target Oligonucleotide sequence Tm (°C) bp
CCSP F: 5'- GAAACTCGCTGTCACCCTCACC-3' 60 211
  R: 5'-TTAATGATGCTTTCTCTGG-3'   
β-actin F: 5'-CCTGTACGCCAACACAGTGC-3'; 60 234
  R: 5'-ATACTCCTGCTTGCTGATCC-3'   
  1. F, forward primer; R, reverse primer; Tm, melting temperature.