Figure 3
Validation of proteomic result (A) Western blotting analysis used to validate the mass spectrometric result. NIH3T3 cells were vehicle or sodium butyrate treated for 4 days followed by isolation of nucleoli. The protein concentration was ascertained and then 10 ug of total protein was run on an SDS-PAGE to compare the levels of B23, NDH II and PARP1 proteins. Western blotting was performed with anti-B23, anti-NDH II and anti-PARP1 to validate the levels of these proteins following sodium butyrate induced senescence. (B) Indirect Immunofluorescence showing B23 localization in control and sodium butyrate treated (4 days) NIH3T3 cells.