Figure 5
Effect of MTX treatment on B23 localization in NIH3T3 cells (A, B) Morphological changes and SA-β-gal staining of NIH3T3 cells when treated with 1 uM MTX for 9 days. The blue coloration is an indication of senescence. (C-H) B23 protein staining in MTX treated NIH3T3 cells. The cells were also counter stained with Hoechst 33258 to stain DNA. The arrows in G point to chromatin foci inside the nucleolus of MTX treated cells. The bars represent 10 μm.