Figure 6

Comparison of the distribution of B23 protein in primary fibroblast cells from lamin -/- and lamin +/+ mice (A) lamin +/+ and lamin -/- cells were lysed in RIPA buffer and Western blotting performed with antibody for lamin A/C, B23 or actin. The lysates were quantitated using Bradford-Lowry reagent and then 10 ug of lysate was run on a SDS-PAGE. (B-K) lamin A +/+ and lamin A-/- cells were grown on coverslips and then stained for B23 protein. The cells were also counter stained with Hoechst 33258 to stain DNA. The arrows in (E) and (J) point to the representative nucleolar staining of B23 in Lamin A +/+ and Lamin A -/- cells respectively. The bars represent 10 μm.