Figure 4
Expression profiles of angiogenesis- and matrix remodeling-related genes in cultured EPCs and decidual cells. EPCs and decidual cells were isolated from decidual capsules at 7.5 days p.c. Decidual cells (5 × 105 cells/well in 6-multiwell plates) and EPCs (10 EPCs/well in 6-multiwell plates) were cultured in DMEM containing 10% FBS for 2-5 days, respectively. Total RNA was isolated, reverse-transcribed, and the indicated sets of genes associated with angiogenesis (left panel) and tissue remodeling (right panel) were analyzed by RT-PCR (25 cycles for VEGF-A, Flk-1, Flt-1, MMP-3, MMP-9, MMP-14, TIMP-1, TIMP-2, TIMP-3; 30 cycles for FGF-2, FGFR-1, IGF-I, IGF-IR, MMP-1). GAPDH (25 cycles) was used as an internal control. The data are representative of three independent experiments.