Table 1 Primers for RT-PCR analysis
Genbank acc no. | Gene | 5'-Forward primer-3' | 5'-Reverse primer-3' | Size (bp) |
|---|---|---|---|---|
NM_010701 | ChM-I | CATCGGGGCCTTCTACTTCT | CTGCGTCGTCCTGAACATTGGG | 584 |
NM_010088 | Prl8a2 | TGAATGTCAAACAGGAGAGAA | CAATCTTGCCCAGTTATGCGG | 628 |
NM_008864 | Prl3d1 | GCTTCCATCCATACTCCAG | TCGTTCTGAAAGACAACTCG | 419 |
NM_009309 | Brachyury | CTGCAGTCCCATGATAACTGGTCTAGC | CCAGGATTTCAAAGTCACATATATGTTGTAG | 717 |
NM_009931 | Col4a1 | TGTCCAAGGCAACGAGCGTG | TGTTCTTCTCATGCACACTTGG | 583 |
NM_007742 | Col1a2 | GAACGGTCCACGATTGCATG | GGCATGTTGCTAGGCACGAAG | 401 |
NM_001025250 | VEGF-A | CAGAGAGCAACATCACCATGC | TCCTCGAAGGATCTCCTCTTCC | 313, 445 |
NM_010612 | Flk-1 | CACAGACACCACCGTGTACTCC | CTCCAAGGTAGACAGACTCGGC | 516 |
NM_010228 | Flt-1 | TATGGTCTGTGTGCTTAGGTCG | ACAGGACCATCTATGGTCTTCC | 476 |
NM_008006 | FGF-2 | AGCGGCATCACCTCGCTTCC | TGGAAGCAGTATGGCCTTCTGTCC | 433 |
NM_010206 | FGFR-1 | AGAGACCAGCTGTGATGA | GGCCACTTTGGTCACACG | 461 |
NM_010512 | IGF-I | GGACCAGAGACCCTTTGCGGGG | GGCTGCTTTTGTAGGCTTCAGTGG | 210 |
NM_010513 | IGF-IR | ATGCTGTTTGAACTGCAGCGCATGTGCTGG | CCGCTCGAGCTTGCGGCCCCCGTTCAT | 354 |
NM_010809 | MMP-3 | GAAATGCAGAAGTTCCTCGG | GAGTTCCATAGAGGGACTGA | 878 |
NM_013599 | MMP-9 | TGTCATCCAGTTTGGTGTCG | TAGGGCAGAAGCCATACAGT | 433 |
NM_008607 | MMP-13 | TGAAGAGACTGAGCGCTGCG | ATTCTTCCATGTGGTTCCAGCC | 461 |
NM_008608 | MMP-14 | ATCATTGAGGTGGATGAGGAGG | CAGCCAACCAGGTACACTTGG | 614 |
NM_001044384 | TIMP-1 | AGTAAGGCCTGTAGCTGTGCC | ACGAGGACCTGATCCGTCC | 467 |
NM_011594 | TIMP-2 | AGCAGTGAGCGAGAAGGAGG | TCACTTCTCTCGATGCAGGC | 450 |
NM_011595 | TIMP-3 | GCTACTGCAGCTGGTACCG | TTGGACTTCTGCCAACTTCC | 518 |
NM008084 | GAPDH | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA | 452 |