Polar diagrams representing the directedness of cathode (Calvaria)- and anode (SaOS-2)-directed cells in the presence of an applied EF or an EF in combination with the NHE3-, NHE1-, or NaKA-specific inhibitors, S3226, HOE 642, or oubain, respectively. FAK inhibition was used as a positive control. Each diagram represents the data from 25-45 cells from three to five individual experiments. DIC images showing the cell shape, morphology, and orientation in the presence of EF only or with inhibitors. Bars: 50 μm.