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Figure 2 | BMC Cell Biology

Figure 2

From: Sirtuin-mediated nuclear differentiation and programmed degradation in Tetrahymena

Figure 2

Extra nuclei in NAM-treated cells acquire new macronucleus characteristics. Cells were mixed in the absence (0 mM NAM) or presence (25 mM NAM) of nicotinamide and fixed with 2% paraformaldehyde at either 10 or 24 hrs into conjugation (post-mixing). Fixed cells were subjected to immunoblot analysis with a α-MLH (micronuclear linker histone; note that for the 24 hr NAM-treated sample a cell with an unusual α-MLH-positive micronucleus is shown for signal comparison), b α-Nop52 (brackets and arrows indicate examples of nuclear bodies that do not bind α-Nop52; "An", anlagen), or c α-acetylated H4 (α-AcH4) antisera (arrows indicate nuclear bodies that bind α-AcH4). All fixed cells were counterstained with DAPI.

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