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Figure 3 | BMC Cell Biology

Figure 3

From: Sirtuin-mediated nuclear differentiation and programmed degradation in Tetrahymena

Figure 3

Nicotinamide prevents degradation of the parental macronucleus during conjugation. Just prior to mixing, cells were exposed to either 0 mM or 25 mM nicotinamide (NAM). At 10 hrs into conjugation (post-mixing), the cells were fixed in 2% paraformaldehyde, exposed to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) reagent to assay for degraded DNA, and stained with DAPI prior to imaging. In cells exposed to NAM, the old macronuclei "OM" were TUNEL negative, while the OM of nontreated cells were TUNEL positive (green). "m", micronucleus. Note: A cell with an unusual TUNEL-positive micronucleus is shown for signal comparison in the NAM-treated sample.

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