Figure 7
From: Sirtuin-mediated nuclear differentiation and programmed degradation in Tetrahymena
Thd14 resides in a large aggregate during starvation. a A starved live cell expressing GFP-Thd14 visualized by fluorescence microscopy. The macronucleus is within the circled regions. Staining outside the nucleus is in food vacuoles. White arrow points to a DAPI poor region where GFP-Thd14 aggregates. "GFP con" is the negative control expressing only GFP (no fusion with Thd14). b DAPI, GFP and DIC images of a macronucleus in a live cell. Masses inside the macronucleus observed by DIC microscopy (arrow) are ringed by GFP-Thd14 and stain weakly with DAPI, both observed by fluorescence imaging. c A macronucleus showing Nop52 ringing around the large aggregate of GFP-Thd14 by immunofluorescence.