Figure 4

PTEN dynamics (green) linked to the propagation of actin waves (red) in a cell recorded over a period of 21 minutes. Within this time the cell undergoes five cycles of wave expansion and retraction. Selected frames of the time series are arranged such that the PTEN entry into the substrate-attached area is aligned in the top row of each period. Consecutive frames of a full period of retraction and expansion of actin waves are ordered in vertical columns below the respective frames of the top row. This arrangement shows that the starting site of each PTEN entry is located roughly opposite to the previous one. Within each period, the inner territory circumscribed by an actin wave shrinks to a minimal size and expands thereafter until it covers the entire substrate-attached membrane. Before PTEN ingresses again, the actin distribution assumes a toroid-like shape by the decline of polymerized actin in the central region. Time is indicated in seconds. Bar, 10 μ m.