Figure 7

Change of spatiotemporal PTEN pattern from circulation to alternation. A cell expressing GFP-PTEN (green) and mRFP-LimEΔ (red) has been incubated with 2 μM latrunculin A and recorded for more than one hour. PTEN started to circulate, but subsequently turned into an alternating mode of lateral ingression into the substrate-attached area of the cell surface. A, image at the beginning of recording, depicting the crescent of PTEN. The directions of views I and II in (B) are shown, and the positions of scans 1 and 2 in (C) are indicated as white circles. Bar, 10 μ m. B, temporal pattern evolution. The spatial pattern is reconstructed along the time axis from top to bottom. The first part of the recording is shown, comprising the turn from circulation to alternation (arrowhead). Contributions of fluorescence from an adjacent cell have been eliminated in this graph. (The border of this second cell is seen in Additional file 10). C, periodic peaks of the GFP-PTEN fluorescence at the two positions indicated in (A). Since these positions differ by 180°, phases of the oscillations are accordingly shifted. D, number of completed periods plotted over time determined at position 1 (triangles) or position 2 (circles). The slope of the curves does not indicate an increase in frequency. It rather suggests a slight decrease after the turn from circulation to alternation. (The small peaks seen in (C) at 2894 and 3720 s have been disregarded).