Figure 2

Nuclear speckles remain aggregated within the nuclei of newly hydrated microspores. a-i, k, and l, microspores were fixed and sectioned after 30 minutes of development. a-d, dual detection of (a) SC35 (red) and (b) Poly(A)+ RNA (green). c, DAPI (blue). d, Merge of a-c. e-h, Dual detection of (e) U2B" (red) and (f) Poly(A)+ RNA (green). g, DAPI (blue). h, Merge of e-g. i, Microspore labeled with monoclonal antibody 72B9 against Fibrillarin (red) and counter stained with DAPI (blue). j, Monoclonal antibody 72B9 labeling (red) of the nucleolus in a sporophytic cell, counter stained with DAPI (blue) and overlain on the phase contrast image (green) of the gametophyte. k, Representative 5S rRNA ISH labeling of the cytoplasm of a microspore (dark staining). l, Representative 25S rRNA ISH labeling of the cytoplasm of a microspore (dark staining). Bar = 25 μm.