Figure 4From: Protein release through nonlethal oncotic pores as an alternative nonclassical secretory pathwayPGK1 released as a free soluble protein. Media was collected from HeLa cell cultures 7.5 min after serum withdrawal and then treated with (lanes 2 and 4) or without Proteinase K (PK, lanes 1 and 3) in the presence (lanes 3 and 4) or absence (lanes 1 and 2) of Triton X-100 (Triton) as described in Materials and Methods. Samples were separated on SDS-PAGE gels and then immunoblotted with anti-PGK1 antibodies.Back to article page