Figure 6

Confocal images of PGK1 at the cell surface. HeLa cells were grown in MEM supplemented with 10% NBS. After 24 h, they were either left untreated (a-c), washed once with serum-free MEM and then incubated with the same media for 30 min (d-f), or washed once with serum-free MEM containing BSA and then incubated with the same media for 30 min (g-i). After the incubations the cells were fixed as described in Materials and Methods. Cells were not permeabilized with detergents in order to detect only cell surface proteins. The cell surface was detected using wheat germ agglutinin (WGA, red) and PGK1 (green) was localized using affinity purified rabbit anti-human PGK1 antibody. The bar represents 10 μm (i).