Figure 2

Identification of SP and NSP fractions in Msi1^high cell population by FACS. (A): A part of cells in Msi1^high cell population (EGF) and control groups (SF and FCS) were presented a relative weak staining after Hoechst 33342 dye. (green arrowheads; Bar indicates 50 μm in this panel) (B): FACS analysis showed a typical profile of dual-wavelength FACS of viable cells based on the Hoechst fluorescence. The distinct SP and NSP fractions were presented in all three groups (EGF, SF, and FCS). The detected cell populations in right row were treated with 50 μM verapamil (+Verapamil). (C): Percentage of SP cells in Msi1^high cells and two control groups (SF and FCS) with or without verapamil pretreatment (+Verapamil and -Verapamil). (Bar shows mean ± SE in this panel; n = 3 individual experiments; *P< 0.05 compared with all other groups).