Figure 5

ABCG2 expression in SP and NSP fractions from Msi1^high cells. Total RNA was extracted from SP, NSP, and unsorted Msi1^high cells and analyzed by RT-PCR for ABCG2. (A): RT-PCR analysis for ABCG2. β-actin served as the control. (B): Relative mRNA expression of ABCG2 indicated by the integrated intensity of the corresponding bands in Panels A are shown in this panel. There was no statistical differentiation in ABCG2 expression among these three groups. (Mean ± SE; n = 3 individual experiments).