Interaction between SOX4 and plakoglobin is affected by Wnt3A signaling. A. LNCaP HASOX4 stable cell lines were treated with vehicle, Wnt3A, LMB, or Wnt3A + LMB and anti-HA immunoprecipitations were immunoblotted for the presence of plakoglobin. B, Quantitative analysis of co-IP and Western blots from panel A using the Odyssey® Infrared Imaging System (Li-Cor Bioscience). Fold changes were calculated as the ratio of immunoprecipitated plakoglobin to SOX4 relative to the vehicle control panel (untreat). (n = 3, independent biological replicates performed on separate days; error bars represent SEM; *, p < 0.05; **, p < 0.01 for a one-sided paired t-test) C. (Upper) LNCaP HASOX4 stable cell lines were treated with vehicle, WNT3A, LMB, or WNT3A + LMB and anti-HA immunoprecipitations were performed using separate cytosolic and nuclear fractions. (Lower) Anti-AKT and anti-Lamin A antibodies were used as controls to examine markers of cytosolic and nuclear fractions, respectively, to confirm the purity of each fraction. D. Quantitative analysis of co-IP and Western blots from panel C.