Figure 2From: Dynamic expression of synemin isoforms in mouse embryonic stem cells and neural derivativesImmunofluorescence detection of synemin in mouse ES cells. A, Western blotting showing the specificity of synemin antibodies with proteins extracted from mouse bladder. The antibody anti-synemin H detected one band of 180 kDa, corresponding to synemin H isoform (lanes 1-2), and the anti-synemin H/M detected two bands 150 kDa and 180 kDa that correspond to H and M isoforms (lanes 3-4). GAPDH was used for the loading control (lanes 5-6). B, Diagram illustrating the regions recognized by antibodies anti-synemin H and anti-synemins H/M. C-K, Immunochemistry analysis demonstrating ES cells colonies labeled for Oct-3/4 (C), keratin 8 (F), SSEA-1 (I), synemin M (D, G) and synemin H (J). Synemin M was present with Oct-3/4 (C-E) and keratin 8 (F-H) in pluripotent ES cells. No immunolabelling for synemin H was observed in undifferentiated ES cells expressing SSEA-1 (I-K). Syn: synemin; K8: keratin 8. Bar = 20 μm.Back to article page